Determination of genetic relations among tomato genotypes with issrs molecular markers in Sulaymaniyah region
2016
Ibrahim, A.S.I.
The goal of this study was to investigate the genetic variation among thirty two tomato genotypes (Solanum lycopersicon L.) grown in Sulaymaniyah using ISSR molecular markers technique. This work was carried out in the Yuzuncu Yil University, Agriculture Faculty, Agricultural Biotechnology Department. Eighteen markers were used to estimate the polymorphism among the local tomato genotypes and were discovered to be a useful tool for genotypes identification. The results of ISSR markers, revealed 65 amplified fragments, 50 of them were polymorphic from using 15 primers. The similarity matrix was computed by using Jaccard similarity coefficients, based on polymorphic bands and dendogram established through UPGMA cluster analysis. The dendogram revealed 3 main groups. Genetic similarity (GS) ranged from 0.261 to 0.941 within studied genotypes. The highest similarity was 0.941 for the genotype pairs G7 and G16. However, the lowest similarity index was 0.261 between G15-G24 and G15-G26. Fifteen ISSR markers used to detect DNA polymorphism gave polymorphism percentage for each primer range between 50–100% with a average polymorphism percentage reaching 75.61%. In general ISSR markers could detect high polymorphism, primer ISSR6 and Sola 11 gave the highest polymorphism percentage were 100%, while primer 3F, Sola 5 and Sola 12 did not give any amplification. The mean of PIC (Polymorphic Information Content) value was 0.50 for ISSR markers. The ISSR technology proved useful in describing genetic diversity among tomato genotypes was collected from different geographical around Sulaymaniyah province.
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