?????????????????????????????????????? Phellinus sp.

Peeradon Puckpernchan. Faculty of Liberal Arts and Science. Department of Bioproducts; Arm Unartngam. Faculty of Liberal Arts and Science. Department of Bioproducts; Jintana Unartngam. Faculty of Agriculture at Kamphaeng Saen. Department of Plant Pathology

Biological activities of Phellinus sp. | ?????????????????????????????????????? Phellinus sp.

2021

Peeradon Puckpernchan(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Liberal Arts and Science. Department of Bioproducts) | Arm Unartngam(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Liberal Arts and Science. Department of Bioproducts) | Jintana Unartngam(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Department of Plant Pathology)

In Thailand, the Kratinphiman mushroom, Phellinus sp., has been reported in traditional Thai medicine with different species and common names. However, the identification of species in this genus is not clear: therefore, it is also not understood which one to use in the context of medicine or biologically active substance production. In this study, the active substances inhibited Bacillus subtilis and Streptococcus aureus were studied. The results revealed that the substances extracted from Phellinus sp. with ethyl-acetate could inhibit some strains of B. subtilis and S. aureuss. Phellinus linteus showed the highest inhibitory substance production when compared with streptomycin 400 ppm. Moreover, the separation of putative antibiotics by HPTLC and bio-autographic methods, demonstrated that the proportion of chloroform: methanol: acetic acid: H2O had a polarity value of 0.436. All Phellinus species produce low polarity antibiotics with an Rf value of 0.79. Moreover, P. noxius isolate PKD644 showed that the antibiotics could be clearly differentiated into two groups. Antibiotic production was from 7-36 percent from isolated biological compounds. The results revealed that isolate PKD644 produced the most antibiotics per unit using 2D TLC isolation, and single and co-activities were observed

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