The prevalence of Pneumocystis jirovecii among patients with different chronic pulmonary disorders in Ahvaz, Iran
2016
Elham Aboualigalehdari | Ali Zarei-Mahmoudabadi | Mahnaz Fatahinia | Esmaeil Idani
Background and Objectives: Pneumocystis jirovecii pneumonia (PJP) is a chronic fungal infection that caused by P. ji- rovecii. Disease is more prevalent among the HIV-infected patients. The colonization of pneumocystis in human respiratory system is associated with the airway inflammation and obstruction. The current study was conducted to identify the preva- lence of P. jirovecii among the patients with chronic pulmonary disorders in Ahvaz, Iran.Materials and Methods: In the present study, 115 bronchoalveolar lavage (BAL) specimens were collected from patients. Samples were subjected to Nested-PCR with specific primers. The second PCR products were used for sequencing analysis.Results: Our findings demonstrated that 31(27.0%) of samples were positive for P. jirovecii. Nine patients (29%) have tuberculosis (TB) followed by, 1(3.2%) HIV positive and 21(67.7%) miscellaneous pulmonary disorders. Our results show that there was no significant differences between sex (male:female ratio, 17:14), TB, HIV and P. jirovecii in BAL samples (P>0.05).Conclusion: The current study is the first report from Ahvaz and it showed a relatively high frequency (27%) of P. jirovecii among patients with different pulmonary disorders. In addition Nested-PCR might be reliable technique for diagnosis of P. jirovecii, while the Grocott's methenamine silver (GMS) have a low sensitivity, which only two positive patients were identified.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
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