Detection of Antibodies Against Trypanosoma evansi in Sheep by Indirect ELISA in Rayalaseema Region of Andhra Pradesh

S. Sivajothi; V.C. Rayulu; P. Malakondaiah; D. Sreenivasulu; B.Sudhakara Reddy

Detection of Antibodies Against Trypanosoma evansi in Sheep by Indirect ELISA in Rayalaseema Region of Andhra Pradesh

2014

S. Sivajothi | V.C. Rayulu | P. Malakondaiah | D. Sreenivasulu | B.Sudhakara Reddy

The present research was carried out with an objective to improve the diagnostic tools for detection of antibodies against Trypanosoma evansi infection using indirect enzyme-linked immunosorbent assay (ELISA) in sheep. In this study standardized the Indirect ELISA for detection of T. evansi in sheep. The optimum concentration of antigen, test sera and conjugate were determined as 5µg per well, 1:10 and 1: 4000 dilutions, respectively. 464 serum samples were collected from sheep in different parts of the Rayalaseema region of Andhra Pradesh for screening of T. evansi infection. Out of 464 serum samples 46 (9.91%) were found positive by indirect ELISA.

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