EFFECT OF CIDR AND OVSYNCH PROTOCOLS ON ESTRUS RESPONSE, FERTILITY AND PLASMA PROGESTERONE AND BIOCHEMICAL PROFILE IN TRUE ANOESTRUS CROSSBRED COWS

ABSTRACTA study was carried out in the AMUL milk shed area on 30 postpartum crossbredcows, comprising 20 true anoestrus and 10 normal cyclic cows to evaluate clinical responseto oestrus induction protocols and plasma progesterone and biochemical profile at differenttime intervals post treatment/pot-AI. The oestrus induction response obtained with CIDR andOvsynch protocol in anoestrus cows (10 each) was 100% and 90% with oestrus inductionintervals of 6.00 + 3.22 and 86.67 + 3.33 hrs from PG injection, respectively. The conceptionrates at induced/first oestrus and overall of 3 cycles were 50 and 80% in CIDR 30 and 60%in Ovsynch protocol: and 80% in normal cyclic control cows. The plasma progesterone levelsin CIDR protocol on day 0, 7, 9/10 (AI) and day 21 post-AI were 0.65+0.23, 3.48+0.54,0.48+0.13 and 10.44+1.77ng/ml in conceived and 0.70+0.18, 3.61+1.05, 0.32+0.09 and1.42+0.38 ng/ml in non conceived cows. The corresponding P4 levels in Ovsynch protocolwere 0.28+0.06, 2.98+0.93, 0.48+0.19 and 8.06+1.16 ng/ml in conceived and 0.32+0.07,3.82+1.35, 0.52+0.30 and 1.24+0.71 ng/ml in non-conceived cows. There was significantcariation in P4 profile between periods in both the protocols (P<0.05). The P4 levels inconceived and non-conceived cows of normal cyclic control group on day 0(AI) were0.24+0.04 and 0.41+0.15ng/ml, and on day 21 post-AI 10.48+1.34 nd 0.63+0.11 ng/ml(P<0.01), respectively. The plasma total cholestrol and total protein concentrations inconceived and non-conceived cows of CIDR protocol were 173.62+13.28 and 186.43+8.94mg/dl, and 12.59+0.38 and 11.07+0.06 g/dl, respectively, without significant variation betweensampling days. In Ovsynch protocol, the corresponding calues of cholestrol and protein were128.88+3.74 and 213.11+5.73 mg/dl, and 11.83+0.22 and 12.14+0.13 gdl. The plasma totalcholestrol levels varied significantly between sampling days in non-conceived animals(P<0.05). There was significant variation between conceoved and non-conceived animals intotal cholestrol profile inCIDR protocol and in total proterin in Ovsynch protocol (P<0.01). Innormal cyclic control animals, the choestrol concentrations on day of AI and day 21 post-AIwere 219.47+22.15 and 192.80+16.79 mg/dl and total protein levels 12.35+0.36 and12.76+0.15 g/dl without significant variation (P0.05) between days and between pregnancystatus. It is concluded that both the treatment protocols induce fertile/ovulatory oestrus andimprove conception rates and plasma P4 profile in true anoestrus cows, though there wasno significant infuence on plasma protein and cholestrol profile.Key words: Crossbred cows, Anoestrus, Oestrus induction/synchronization protocols, Fertility,Progesterone, Metabolites, Conceived and non-conceived groups.