Genetic diversity in Stellaria L. (Caryophyllaceae) using sequence related amplified polymorphism
2021
Li, Jialing | Yang, Xin | Mehri, Shahram
Li J., X. Yang, S.Mehri (2021). Genetic diversity in Stellaria L. (Caryophyllaceae) using sequence related amplified polymorphism. - Genetika, Vol 53, No.3, 1369 - 1377. Nearly 120 species exist in the genus Stellaria L. (Caryophyllaceae, Alsinoideae) with a general distribution in the temperate zones of Asia and Europe. Totally, Stellaria exhibits 9 taxa in Iran and sequence-related amplified polymorphism was used to assess their genetic diversity. Therefore, 72 cases of 5 Stellaria were collected in seven provinces. Then, polymerase chain reaction amplifications (PCR) amplification of 5 species of Stellaria was employed for producing 78 (Number of total loci) (NTL) DNA bands. In addition, five selective primers were combined to produce the above bands. Results showed the total number of amplified fragments in ranges between 8 and 15 and variations of the predicted unbiased heterozygosity (H) between 0.22 (S. persica) and 0.39 (S. holostea). Moreover, estimations indicated the genetic similarity between 5 species from 0.73-0.92 and two key clusters were obtained by the clustering results. Considering the analysis of the SRAP markers, the minimum similarity was observed in S. pallida and S. holostea. Furthermore, distance (Mantel test results) showed a considerable signature of isolation. According to the findings, SRAP can detect and decipher genetic affinities in the Stellaria species. Hence, these findings could be used in conservation and biodiversity programs and also provide the ground for choosing appropriate ecotypes for pasture and forage purposes in Iran in the future.
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