Determination of genomic dna quantity and purity of different Geophyte genus

Ecology of our country is very convenient in terms of Geophytes. World geophytes (bulbs, tubers, tuberous root, rhizome plants and so on) are an important part in Flora of Turkey. Geophytes grown in Turkey seventy–eight belonging to the genus containing about 1,027 taxa (species, subspecies, varieties, forms) and approximately 40% of them are endemic. A large number of these species within the Yalova Atatürk Central Horticultural Research Institute are under protection. It is required to isolate DNA high purity and enough quantity to successfully carry out the study of molecular biology. Also considering the possibility of extinction of endemic geophyte species increased the importance of this work. In this study, different Geophyte species collected from Turkey ecology DNA isolated from fresh leaves. DNA isolation and cold storage of the total population was 2,517. The results showed that the modified protocol almost successfully produced a sufficient amount of DNA with high quality. It was obtained high purity and sufficient amounts of DNA from Delphinium, Erodium, Cyclamen, Fritillaria, Allium, Crocus, Primula and Anemone. However sufficient purity and quantity of DNA could not be obtained from most of the Asphodelus, Oxalis ve Asphodeline species. 65% of the isolated DNA results were successful but 35 % of the total samples were obtained poor quality DNA. The average amount of samples in a high–quality DNA isolation from 83 ng/µl, and the purity value was 1.89. In the light of these results, Even if studied excessive number of species, different leaf types and their contents although the obtained results can be said to be successful. In this study, future identification, genetic mapping, molecular research all kinds of similarities to the relationship, the source and origin of the genetic material to be protected, and storage of certain realized.