Opening in vitro of cut shasta daisy bud (Chrysanthemum maximum Ramond) [study conducted in the Philippines]
1981
Rimando, T.J. | Maralit, M.L. (University of the Philippines at Los Banos, College, Laguna. Dept. of Horticulture)
To reduce mechanical damage and prolong vaselife during handling and transport of fully opened cut Shasta daisy (Chrysanthemum maximum Ramond), we determined the feasibility of harvesting it in the bud stage and inducing it to open under artificial conditions. Flowers as early as stage I (25% developed relative to field-opened flowers) were successfully opened in vitro. Artificially opened flowers attained head diameters greater than those allowed to open in the field. Vaselife improved in both field-opened and artificially opened flowers when held in flower preservatives. With 200 ppm 8-hydroxyquinoline sulfate or a mixture of n-alkyl dimethyl benzyl ammonium chloride and n-alkyl dimethyl ethyl benzyl ammonium chloride (Physan 20) and 100 ppm citric acid, the optimum sucrose levels for maximum vaselife for field-opened and in vitro opened flowers were 10% and 12% sucrose, respectively. The rate of flower bud opening increased with increasing temperatures from 5 C to 20 C. Final head diameters of in vitro-opened flowers compared with field-opened flowers were not affected by temperatures as low as 5 C
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