Propagation of Amaryllis through tissue culture
1982
Salak Phansiri
Bulb-scale sections of amaryllis (Hippeastrum hybridum), 8 hybrids and one native variety, were cultured on modified Murashige and Skoog basal medium supplemented with 2, 4-dichlorophenoxyacetic acid (2, 4-D) at 0.3 or 3 ppm. combined with 6-furfurylamino purine (kinetin) at 0 or 0.3 ppm. Five varieties; Caroussel, Excelsior, Scarlet Leader, Rembrandt and Rilona, were able to regenerate bulblets. Excelsior tissuies formed callus 90% and regenerate bulblet 50%. Both Rilona and Caroussel formed callus 70% and bulblet 30%. Regeneration of bulblet in four varieties took 60-70 days but Caroussel variety took 32 days
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