An evaluation of three serological tests for antibody to Brucella suis in pigs
1989
Rogers, R.J. | Ketterer, P.J. | Baldock, F.C. | Blackall, P.J. | Stewart, R.W. (Queensland Dept. of Primary Industries, Yeerongpilly (Australia). Animal Research Inst.) | Cook, D.J. (Queensland Dept of Primary Industries, Rockhampton (Australia). Rockhampton Veterinary Lab.)
The Rose Bengal Plate Agglutination test (RBT), the complement fixation text (CFT) and the tube agglutination test (TAT) were applied to serums from 345 feral and 80 domestic pigs sampled at slaughter. Some 58 feral and 35 domestic pigs were culture-positive for B. Suis. The sensitivity (Se) in culture-positive pigs of the RBT (79.1 percent) was significantly greater than that of either the CFT (49.1 percent) or TAT (51.1 percent). The specificities (Sp) in culture-negative pigs were 81.2 percent for the RBT, 90.8 percent for the CFT and 81.0 percent for the TAT. A more realistic estimate of Sp for the RBT was considered to be 97.6 percent, based on serological results from 31,326 domestic pigs routinely tested for regulatory purposes. The RBT was clearly superior to the other 2 tests in this study. However, a more sensitive screening test would be preferable for use in a test and slaughter eradication program. The RBT would be a suitable confirmatory test.
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