Biopotency in vitro and metabolic clearance rates of five pituitary preparations of follicle stimulating hormone [livestock]

Phillips, D.J.; Hudson, N.L.; Lun, S.; Condell, L.A.; McNatty, K.P.

Biopotency in vitro and metabolic clearance rates of five pituitary preparations of follicle stimulating hormone [livestock]

1993

Phillips, D.J. | Hudson, N.L. | Lun, S. | Condell, L.A. | McNatty, K.P. (Wallaceville Animal Research Centre. Upper Hutt (New Zealand))

Five pituitary preparations of follicle stimulating hormone (FSH), namely NIDDK-oFSH-17, Bioscan oFSH, Ovagen, Folltropin-V, and F.S.H.-P., were examined for biological activity in terms of their potency in an in vitro bioassay, receptor assay and heterologous radioimmunoassay and in terms of their metabolic clearance rates. The results show that a number of widely available FSH preparations have differing biopotencies. Moreover, the biopotency of a preparation in vitro is not related to its metabolic clearance rate, and not all FSH preparations behave identically in different assays. Measures of biopotency in vitro combined with those of metabolic clearance rate may provide useful information on the properties of FSH preparations used for research purposes and for superovulation of farmed livestock.

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