Molecular analysis of the dhp1(+) gene of Schizosaccharomyces pombe: an essential gene that has homology to the DST2 and RAT1 genes Saccharomyces cerevisiae
1994
Sugano, S. (Tokyo Univ. (Japan). Inst. of Medical Science. Dept. of Molecular Biology) | Shobuike, T. | Takeda, T. | Sugino, A. | Ikeda, H.
The DST2 gene of Saccharomyces cerevisiae encodes a DNA strand exchange protein, STPbeta, which is required for homologous recombination in both mitotic or meiotic cells. A DST2-related gene from the fission yeast Schizosaccharomyces pombe was cloned and designated dhp1(+). The nucleotide sequence of dhp1(+) revealed an open reading frame encoding a protein composed of 991 amino acids. The predicted amino acid sequence was significantly homologous to the S. cerevisiae STPbeta, but lacked the carboxy-terminal sequence present in STPbeta. Furthermore, dhp1(+) shows greater homology to RAT1/HKE1, a gene which is involved in RNA trafficking and processing. Genetic experiments showed that dhp1(+) on an S. cerevisiae expression vector could rescue both the defects of the S. cerevisiae DST2 disruptant, slow growth rate and a sporulation defect, and the lethality of the S. cerevisiae rat1(ts) mutation. This implies the functional similarity of dhp1(+) to both DST2 and RAT1. However unlike DST2, dhp1(+) is an essential gene for cell growth in S. pombe, suggesting that dhp1(+) is not the true homologue of DST2 but rather of RAT1 in S. pombe. The possible roles of dhp1(+) in recombination and cell growth in S. pombe are discussed
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل ZB MED Nutrition. Environment. Agriculture