Comparison of the Johne's Absorbed EIA and the complement-fixation test for the diagnosis of Johne's disease in cattle [Mycobacterium paratuberculosis]

Ridge, S.E.; Morgan, I.R.; Condron, R.J. . Animal Science Inst.; Sockett, D.C.; Collins, M.T. . School of Veterinary Medicine; Skilbeck, N.W. . Regional Veterinary Lab.; Webber, J.J. . Regional Veterinary Lab.

Comparison of the Johne's Absorbed EIA and the complement-fixation test for the diagnosis of Johne's disease in cattle [Mycobacterium paratuberculosis]

1991

Ridge, S.E. | Morgan, I.R. | Condron, R.J. (Victorian Dept. of Agriculture, Attwood (Australia). Animal Science Inst.) | Sockett, D.C. | Collins, M.T. (Winsconsin Univ., Madison (USA). School of Veterinary Medicine) | Skilbeck, N.W. (Victorian Dept. of Agriculture, Bairnsdale (Australia). Regional Veterinary Lab.) | Webber, J.J. (Victorian Dept. of Agriculture, Hamilton (Australia). Regional Veterinary Lab.)

A commercially available absorbed ELISA, the Johne's Absorbed EIA, was compared with the conventional complement-fixation test (CFT) used in Australia. The EIA detected 48.8 percent of 43 Australian cattle with subclinical Johne's disease (JD), while the CFT detected only 12 (21.4 percent) of 56 subclinically affected cattle. Of 150 subclinically infected US cattle, the EIA detected 47.3 percent and the CFT 52.0 percent. The EIA detected 59.7 percent of animals shedding M. paratuberculosis in their faeces, but showed no clinical signs of JD, while the CFT detected 57.3 percent. The EIA correctly identified 88.2 percent of 136 histologically confirmed clinical cases, and the CFT detected 83.4 percent. The EIA was found to have a specificity of 99.8 percent when 998 Australian animals were used as the test population, and 99.0 percent when 196 US animals were used. The specificity of the CFT using Australian samples was 96.9 percent and 95.2 percent using US samples.

اظهر المزيد [+]