Chromosome assortment in Saccharum
1994
Al-Janabi, S.M. | Honeycutt, R.J. | Sobral, B.W.S. (California Inst. of Biological Research, La Jolla (USA))
Recent work has revealed random chromosome pairing and assortment in Saccharum spontaneum L., the most widely distributed, and morphologically and cytologically variable of the species of Saccharum. This conclusion was based on the analysis of a segregating population from a cross between S. spontaneum 'SES 208' and a spontaneously-doubled haploid of itself, derived from anther culture. To determine whether polysomic inheritance is common in Saccharum and whether it is observed in a typical biparental cross, chromosome pairing and assortment was studied in 44 progeny of a cross between euploid, meiotically regular, 2n = 80 forms of Saccharum officinarum 'LA Purple' and Saccharum robustum 'Mol 5829'. Papuan 2n 80 forms of S. robustum have been suggested as the immediate progenitor species for cultivated sugarcane (Saccharum officinarum). A total of 738 loci in LA Purple and 720 loci in Mol 5829 were amplified and typed in the progeny by arbitrarily primed PCR using 45 primers. Fifty and 33 single-dose polymorphisms were identified in the S. officinarum and S. robustum genomes, respectively (chi square at 98%). Linkage analysis of single-dose polymorphisms in both genomes revealed linkages in repulsion and coupling phases. In the S. officinarum genome, a map hypothesis gave 7 linkage groups with 17 linked and 33 unlinked markers. Four of 13 pairwise linkages were in repulsion phase and 9 were in coupling phase. In the S. robustum genome, a map hypothesis gave 5 linkage groups, defined by 12 markers, with 21 markers unlinked, and 2 of 9 pairwise linkages were in repulsion phase. Therefore, complete polysomic inheritance was not observed in either species, suggesting that chromosomal behavior is different from that observed by linkage analysis of over 500 markers.
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تم تزويد هذا السجل من قبل ZB MED Nutrition. Environment. Agriculture