A comparison of the nucleotide sequence of the cerato-ulmin gene and the rDNA ITS between aggressive and non aggressive isolates of Ophiostoma ulmi sensu lato, the causal agent of Dutch elm disease

Jeng, R.; Hintz, W.E.; Bowden, C.G.; Horgen, P.A.; Hubbes, M. . Earth Science Center. Faculty of Forestry

A comparison of the nucleotide sequence of the cerato-ulmin gene and the rDNA ITS between aggressive and non aggressive isolates of Ophiostoma ulmi sensu lato, the causal agent of Dutch elm disease

1996

Jeng, R. | Hintz, W.E. | Bowden, C.G. | Horgen, P.A. | Hubbes, M. (Toronto Univ. (Canada). Earth Science Center. Faculty of Forestry)

Little genetic information exists comparing aggressive and non-aggressive isolates of the causal agent of Dutch elm disease, Ophiostoma ulmi. Two genetic elements were compared between the subgroups. The cerato-ulmin cu gene product has been associated with disease symptoms. Nucleotide-sequence analysis of cu and the internal transcribed spacer (ITS) region of the rDNA were made from three aggressive and three non-aggressive isolates of the pathogen. The results suggested uniformity within, and unique differences between, subgroups. Differences were detected for cu in the promoter, coding, and transcription termination regions. Sequence data for the ITS clearly distinguish the subgroups.

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