Influence of bacterial antigens on specific and non-specific immune response in bester (Huso huso x Acipenser ruthenus) fry F3
1999
Kolman, H. (Inst. of Biology and Environmental Protection, Olsztyn (Poland). Div. of General Biology) | Kolman, R. | Siwicki, A. K.
The study demonstrates the effects of antigens on specific and non-specific immune response in bester (Huso huso x Acipenser ruthenus). The examinations were carried out on bester F3, age 0+ and initial mean b. w. 100 g, reared in aquacultural conditions. The experimental fishes were intraperitoneally injected: Aeromonas salmonicida bacterin at a dose of 10 mg in 0.2 ml PBS/fish as well as Furovac vaccine at a dose of 0.2 ml/fish, the control fishes were injected with PBS. During 6 weeks after antigen application the fishes were bled and analytical studies were conducted. The statistical significance of differences between average levels of assayed indices in experimental and control fishes confirmed in NBT-test, titres of natural and specific antibodies as far as gamma-globulin levels and total protein content, lysozyme activity and phagocytar index of the neutrophile granulocytes. It was found that bacterins stimulated non-specific and specific immunity. The Furovac vaccine was more immunogenic for bester than O-antigen bacterin.
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