Composition of mycoflora and aflatoxins in lupine seeds from the Sudan [Lupinus termis Forrsk.]
2000
El-Nagerabi, S.A.F. (Taiz Univ. (Yemen). Dept. of Microbiology) | Elshafie, A.E. (Sultan Qaboos Univ., Muscat (Oman). Dept. of Biology)
إنجليزي. Thirteen seed samples of lupines (Lupinus termis Forrsk.) were evaluated for their phytosanitaty status by incubation on potato-dextrose agar (PDA) and moist sterile filter papers (blotter method) at 28 +-2 deg C. The seeds were also assayed for the presence of aflatoxins and toxigenic fungi. Forty-seven species and 10 varieties in 14 genera were recovered. Among these, 45 species and 10 varieties were new for lupine seeds and 5 species and 2 varieties were new to the mycoflora of the Sudan. The genus Aspergillus (10 species, 8 varieties) was the most common, followed by Rhizopus (1 species), Fusarium (6 species) and Alternaria (5 species), while the remaining genera (Chaetomium, Cladosporium, Curvularia, Drechslera, Penicillium, Phoma, Emericella, Mucor, Sclerotium, Ulocladium) displayed lower levels of contamination. Of possible pathogens on lupine plants, Alternaria (5 species) (stem lesion) and F. oxysporum (root rot) were recovered from the seeds. Thin-layer chromatographic analysis of chlorophorm extracts of the 13 seed samples revealed that two samples contained very low concentration of aflatoxins B1 and B2 (4.5 microng/kg)
اظهر المزيد [+] اقل [-]إيطالي. [E' stato valutato lo stato sanitario di tredici campioni di semi di lupino (Lupinus termis Forrsk.) mediante incubazione su agar patata-destrosio (PDA) e carte da filtro sterili umide (metodo del tampone) a 28 +-2 gradi C. E' stata pure saggiata la presenza di aflatossine e di funghi tossigeni. Sono state individuate 47 specie e 10 varieta' appartenenti a 14 generi. Fra queste, 45 specie e dieci varieta' erano nuove per i semi di lupino e 5 specie e 2 varieta' risultavano nuove per la micoflora del Sudan. Il genere Aspergillus (10 specie, 8 varieta') era il piu' comune, seguito da Rhizopus (1 specie), Fusarium (6 specie) e Alternaria (5 specie), mentre i generi rimanenti (Chaetomium, Cladosporium, Curvularia, Drechslera, Penicillium, Phoma, Emericella, Mucor, Sclerotium, Ulocladium) presentavano minori livelli di contaminazione. Dei possibili patogeni delle piante di lupino, dai semi sono stati ritrovati Alternaria (5 specie) (lesione del fusto) e Fusarium oxysporum (marciume radicale). L'analisi cromatografica su strato sottile di estratti in cloroformio dei 13 campioni di semi ha evidenziato che due campioni presentavano una concentrazione molto bassa di aflatossine B1 e B2 (4,5 microng/kg)]
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
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