Molecular identification of phytoplasmas from dill (Anethum graveolens L. - Umbelliferae) [Liguria]

إنجليزي. Dills (Anethum graveolens L.) grown from seeds in a small area of the Italian Riviera showed symptoms including stalk discoloration, flower virescence and phyllody. The affected plants were tested for the presence of phytoplasmas. DNAs were PCR-amplified in reactions with ribosomal universal or group-specific primer pairs and analysed by restriction digestions with BfaI or MseI enzyme. Phytoplasmas, identified as members of 16SrI-B, I-C, V-A and XII-A subgroups, were present in 18 out of 22 samples (81.8%). Of these, 9 (50% of the infected) carried a single phytoplasma (3 subgroup 16SrI-B (16.6%), 1 16SrI-C (5.5%), and 5 16SrV-a (27.7%)). Seven samples (38.8%) carried two different phytoplasmas (6 members of subgroups V-A and XII A; 1 member of subgroups I-C and V-A). Two samples carried phytoplasmas of three different subgroups in different mixes (I-C, V-A and XII-A; I-B, I-C and XII-A). There was no clear correlation between a given symptom and the presence of one or more phytoplasmas. This is the first report from Europe of phytoplasmas in dill plants

إيطالي. [Piante di aneto (Anethum graveolens), derivate da seme in una piccola area della riviera ligure italiana, mostravano sintomi quali decolorazione del fusto, virescenza dei fiori e filloidia. Le piante affette sono state testate per la presenza di fitoplasmi. Il DNA e' stato amplificato con PCR. I fitoplasmi, identificati come membri dei sottogruppi 16SrI-B, I-C, V-A e XII-A, erano presenti in 18 dei 22 campioni (81,8%). Di questi, 9 (50% degli infettati) portavano a un singolo fitoplasma. Sette campioni portavano a due differenti fitoplasmi. Due campioni presentavano fitoplasmi di tre differenti sottogruppi in combinazioni diverse. Non si e' riscontrata una chiara correlazione tra un dato sintomo e la presenza di uno o piu' fitoplasmi. Questa e' la prima nota in Europa su fitoplasmi in piante di aneto]