Biotechnology for leaf quality of lettuce [Lactuca sativa L.]
2003
Giannino, D. | Frugis, G. | Nicolodi, C. | Santamaria P. | Mariotti, D. (Consiglio Nazionale delle Ricerche, Monterotondo, Rome (Italy). Istituto di Biologia e Biotecnologie Agrarie) | Santini, L. (Bari Univ. (Italy). Dipartimento di Scienze delle Produzioni Vegetali)
إنجليزي. Genetic transformation of lettuce by A. tumefaciens has been standardised and manipulation of traits such as leaf shape and nitrogen metabolism has been carried out. Lettuce (cv Luxor) overexpressing the homeobox knotted-like gene KNAT1 of A. thaliana under the pea plastocyanin promoter featured leaves with increased vascular net, lobes at margins and higher content of cytokinin hormones, which delayed senescence, as compared to wild types. Lettuce overexpressing the asparagine synthetase A from E. coli under a chimaeric promoter derived from 35S and mannopine synthase promoters exhibited accelerated growth, higher fresh and dry weight and reduced content of nitrates compared to untransformed plants. Finally, from open field trials, pollen transmission was estimated very poor (1%) even when donor and recipient plants were in close proximity
اظهر المزيد [+] اقل [-]إيطالي. Il trasferimento genico mediante Agrobacterium tumefaciens ha consentito la manipolazione mirata dei tratti fogliari e del metabolismo azotato in lattuga. La cultivar Luxor, che esprime ectopicamente il gene omeotico KNAT1 di Arabidopsis thaliana, ha presentato foglie con margini alterati e un incremento di citochinine, che ritarda la senescenza, rispetto ai controlli. Le lattughe Cortina, che sintetizzano l'enzima asparagina sintetasi A del batterio E. coli, hanno mostrato crescita accelerata, maggior peso e minor contenuto di nitrati rispetto ai controlli. Infine, in esperimenti di pieno campo, si e' osservato che la percentuale di trasmissione dei transgeni via polline era inferiore all'1%.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Istituto di Servizi per il Mercato Agricolo Alimentare