Molecular identification of Cordylobia anthropophaga Blanchard (Diptera: Calliphoridae) larvae collected from dogs (Canis familiaris) in Jos South, Plateau State, Nigeria
2012
Ogo, Ndudim I.(National Veterinary Research Institut ,Instituto de Investigacion en Recursos Cinegiticos) | Onovoh, Emmanuel(National Veterinary Research Institut) | Okubanjo, Oluyinka O.(Ahmadu Bello University Department of Veterinary Parasitology) | Galindo, Ruth C.(Instituto de Investigacion en Recursos Cinegiticos) | Lastra, Jose-Manuel P. de la(Instituto de Investigacion en Recursos Cinegiticos) | Fuente, Jose de la(Instituto de Investigacion en Recursos Cinegiticos)
Myiasis-causing larvae were extracted from dogs attending veterinary clinics in Plateau State, Nigeria and subjected to molecular analysis involving polymerase chain reaction amplification of the 28S rRNA gene of blowflies, cloning and sequencing techniques. All larvae were confirmed as Cordylobia anthropophaga Blanchard (Diptera: Calliphoridae) after the initial morphological identification. This is the first molecular identification of any myiasis-causing fly species in Nigeria and may serve as a reliable alternative to morphological identification where samples are not well preserved or difficult to identify to species level.
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