Alkali extraction of beta-D-Glucans from Saccharomyces cerevisiae cell wall and study of their adsorptive properties toward zearalenone
2004
Yiannikouris, Alexandros | François, Jean | Poughon, Laurent | Dussap, Claude-Gilles | Bertin, Gérard | Jeminet, Georges | Jouany, J.P. | Unité de Recherches sur les Herbivores (URH) ; Institut National de la Recherche Agronomique (INRA) | Unité mixte de recherche biotechnologies bioprocédés ; Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse) ; Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS) | Centre Universitaire des Sciences et Techniques ; Partenaires INRAE | Alltech-France | Université Blaise Pascal - Clermont-Ferrand 2 (UBP) | Synthèse et étude de systèmes à intêret biologique (SEESIB) ; Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)
International audience
اظهر المزيد [+] اقل [-]إنجليزي. The isolated cell wall of Saccharomyces cerevisiae has some capacity to adsorb zearalenone (affinity near 30%) and reduce the bioavailability of toxins in the digestive tract. The adsorption process was quantified in vitro, and the data obtained when plotted with Hill's equation indicated a cooperative process. The model showed that the adsorption capacity was related to the yeast cell wall composition. This work focused on the role of various beta-D-glucan types in the efficacy of zearalenone adsorption by yeast cell wall and sought to elucidate some of the adsorption mechanisms. Zearalenone was mixed at 37 degreesC with a constant quantity of alkali-soluble or alkali-insoluble beta-D-glucans isolated from yeast cell walls, and the amount of adsorbed zearalenone was measured. Given that the alkali solubility of beta-D-glucans is a determining factor for their three-dimensional conformation and that the alkali-insoluble fraction had a greater affinity (up to 50%) than the alkali-soluble fraction (similar to16%), it was concluded that the three-dimensional structure strongly influences the adsorption process. The alkali insolubility of beta-D-glucans led to the formation of single and/or triple helices, which have been identified as the most favorable structures for zearalenone adsorption efficacy. The beta(1,3)-D-glucan and beta(1,6)-D-glucan compositions of the two alkali-extracted fractions and their involvement in the adsorption process are discussed.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique