Quantitative trait loci (QTL) identification in the progeny of a polycross
2016
Pauly, Laurence | Flajoulot, Sandrine | Garon, Jerome | Julier, Bernadette | Beguier, Vincent | Barre, Philippe | Jouffray-Drillaud | GIE GRASS | Unité de Recherche Pluridisciplinaire Prairies et Plantes Fourragères (P3F) ; Institut National de la Recherche Agronomique (INRA)
Connected multiparental crosses are valuable for detecting quantitative trait loci (QTL) with multiple alleles. The objective of this study was to show that the progeny of a polycross can be considered as connected mutiparental crosses and used for QTL identification. This is particularly relevant in outbreeding species showing strong inbreeding depression and for which synthetic varieties are created. A total of 191 genotypes from a polycross with six parents were phenotyped for plant height (PH) and plant growth rate (PGR) and genotyped with 82 codominant markers. Markers allowed the identification of the male parent for each sibling and so the 191 genotypes were divided into 15 full-sib families. The number of genotypes per full-sib family varied from 2 to 28. A consensus map of 491 cM was built and QTL were detected with MCQTL-software dedicated to QTL detection in connected mapping populations. Two major QTL for PH and PGR in spring were identified on linkage groups 3 and 4. These explained from 12% to 22% of phenotypic variance. The additive effects reached 12.4 mm for PH and 0.11 mm/C degrees d for PGR. This study shows that the progenies of polycrosses can be used to detect QTL.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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تم تزويد هذا السجل من قبل Institut national de la recherche agronomique