Characterisation of South African field Ehrlichia ruminantium using multilocus sequence typing
2023
Dlamkile, Zinathi(University of Pretoria Faculty of Veterinary Science Department of Veterinary Tropical Diseases) | Neves, Luis(University of Pretoria Faculty of Veterinary Science Department of Veterinary Tropical Diseases,Eduardo Mondlane University Centro de Biotecnologia) | Morar-Leather, Darshana(University of Pretoria Faculty of Veterinary Science Department of Veterinary Tropical Diseases) | Brandt, Christopher(Agricultural Research Council Onderstepoort Veterinary Research Institute Department of Vaccines and Diagnostics Development) | Pretorius, Alri(University of Pretoria Faculty of Veterinary Science Department of Veterinary Tropical Diseases,Agricultural Research Council Onderstepoort Veterinary Research Institute Department of Vaccines and Diagnostics Development) | Steyn, Helena(Agricultural Research Council Onderstepoort Veterinary Research Institute Department of Vaccines and Diagnostics Development) | Liebenberg, Junita(Agricultural Research Council Onderstepoort Veterinary Research Institute Department of Vaccines and Diagnostics Development)
Heartwater, one of the major tick-borne diseases of some domestic and wild ruminants in Africa, is caused by Ehrlichia ruminantium. The genetic diversity of E. ruminantium isolates renders the available vaccine ineffective against certain virulent isolates. To better understand the E. ruminantium genotypes in South Africa, a total of 1004 Amblyomma hebraeum tick deoxyribonucleic acid (DNA) samples from cattle in three South African provinces were tested by pCS20 Sol1 real-time polymerase chain reaction (qPCR) and characterised by multilocus sequence typing (MLST) using five housekeeping genes. Out of 1004 samples tested, 222 (22%) were positive for E. ruminantium. The occurrence of E. ruminantium in Mpumalanga, KwaZulu-Natal and Limpopo provinces was 19%, 22% and 27%, respectively. The E. ruminantium positive samples were screened for housekeeping genes and sequenced. Phylogenetic analysis revealed three main lineages: clade 1 made up of worldwide isolates (eastern, southern Africa, and Caribbean isolates), clade 2 comprised only West African isolates and clade 3 consisted of Omatjenne, Kümm2 and Riverside. Some study sample sequences were not identical to any of the reference isolates. However, they could all be grouped into the worldwide clade. Genetic variation in the sequenced regions was observed in the form of single nucleotide polymorphisms (SNPs). Using MLST to characterise E. ruminantium field isolates allowed the South African genotypes to be clearly distinguished from the distinct West African isolates. CONTRIBUTION: Characterisation of E. ruminantium field isolates is important for the control of heartwater and contributes to preliminary knowledge required for the development of a more practical vaccine against heartwater.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Scientific Electronic Library Online South Africa