Molecular evaluation of genetic diversity and genetic analysis of Azerbaijan sweet cherries (Prunus avium L.) using capillary electrophoresis with fluorescencelabeled SSR markers
2023
Akparov, Zeynal | Mukhtarova, Sheker
The sweet cherry (Prunus avium L.) is one of the world’s most commercially important perennial crops and its improvement has been the focus of human effort for thousands of years. A collection of 74 accessions of Prunus avium L. from a research station, across regions in Azerbaijan representing the sweet cherry germplasm in the country, were examined to estimate genetic diversity and to identify genetic relationships among accessions using a set of 12 microsatellite (SSR) markers. Among the primers used, PaCITA 18, pchgmS 2, AK 193, PaCITA 14A, PaCITA 14B, and AK 200 are polymorphic and effective enough to differentiate between cherry genotypes. After calculating the genetic similarity index, a genetic link dendrogram was constructed using phylogenetic and structure analysis. We found the highest number of alleles at the AK 193 locus (12). The PIC value ranged from 0.0322 to 0.7753. Genetic diversity ranged from 0.0312 to 0.8016. We recorded the highest index of genetic diversity for the AK 123 locus. Thus, the results show that Azerbaijan germplasm has good potential for cherry genotypes for further breeding studies. The information obtained from SSR fingerprinting will be useful in optimizing the conservation of sweet cherry genetic resources present in the Azerbaijan region. However, this approach can be applied to optimize the conservation of local genetic resources of other stone fruit tree species.
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