Molecular characterization and grain quality of identified high iron transgenic i64 events with overexpressed OsNAS2 and SoyferHI genes
2018
Dueñas, C.N. Jr
Events from two constructs with simultaneous constitutive expression of Nicotianamine synthase 2 gene using CaMV35s promoter and Soybean ferritin gene controlled either by, Glubulin-B1 (IRS493) or Glutelin-A2 (IRS495) were screened for candidate events based on high iron, single T-DNA copy and homozygous lines. Based on screening, homozygous T2 lines IR64-IRS493-112-1 and IR64-IRS495-301-7 were identified from TO events with more than 8 ppm and have single copy of T-DNA. Flanking sequence analysis showed disruption in the promoter region of BGOIOSGA009311 in Chromosome 2 for IR64-IRS495-301-7of which may have caused yield, penalty. While IR64-IRS493-112-1had disruption in the promoter region of BGIOSG4A14560 in Chromosome 4 but was off-set by 4 alternative genes with conserved Aminotransferase IV motif found in BGIOSG4A14560. Iron and zine level in T3 homozygous polished seeds from IR64- IRS495-301-7 reached 14ppm and 58ppm respectively while 10ppm and 39ppm for IR64- IRS493-112-1. Range of the values among the grain quality indicators showed no substantial change in IR64 qualities in both lines. IR64-IRS493-112-1 is a very promising candidate based results of this study and for further characterization and possible deregulation as a product that will aid in alleviation of iron and zine deficiency identified to be prevalent globally.
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