Effect of pre and post inoculation of amino acids on induction of resistance and polyphenol oxidase activity against Albugo candida in mustard
2013
Tirmali, A.M. | Kolte, S.J.
In the present study, the mustard genotype EC-399301 (RESJ-177) (white rust- susceptible at cotyledonary leaf stage but resistant at the true leaf stage), when treated with the amino acids at the cotyledonary leaf stage following pre inoculation application of A. candida showed no development symptoms in contrast to profuse development of white rust (WR) pustules on cotyledons of similarly inoculated check plant of the same genotype. The results thus revealed induction of host resistance of otherwise susceptible cotyledonary stage of EC-399301 (RESJ-177). No symptom development due to the amino acids was characterized by expression of hypersensitive-like necrotic lesions on the cotyledons in T₁ L-Pro (@1000 ppm), T₇ L-Glu acid (@1000 ppm), T₉ L-Cys Hyd (@1500 ppm) treatments. The significant minimum Polyphenol oxidase activity was recorded in treatment T₅ L-Cys (@2000 ppm (0.125 unit/mg), in comparison to check (0.156 unit/mg). Following post inoculation application of A. candida no symptom development due to the amino acids was characterized by expression of hypersensitive-like necrotic regions on the cotyledons in all treatments of amino acids @ 2000 ppm except T₃ L-Tyr. The Polyphenol oxidase activity was recorded in the range of 0.048 to 1.520 unit/mg in comparison to check (0.156 unit/mg). In post inoculation application of the above amino acids, hypersensitive response of the treated cotyledons was associated with concomitant increase in the polyphenol oxidase activity. The results thus revealed induction of host resistance of otherwise susceptible cotyledonary stage of EC-399301 (RESJ-177).
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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