Occurrence of Campylobacter spp., Salmonella spp. and shiga toxin‐producing Escherichia coli in inline milk filters from Swedish dairy farms
2020
Flink, Catarina | Nyberg, Karin
This study investigated the occurrence of shiga toxin‐producing Escherichia coli (STEC), thermotolerant Campylobacter spp. and Salmonella spp. in Swedish dairy milk. A total of 302 inline milk filters were analyzed. Salmonella was not isolated from any filters. Polymerase chain reaction screening detected thermotolerant Campylobacter in 30.5% of the milk filters analyzed and it was isolated from 12.6% of filters. The stx genes (stx ₁, stx ₂, or both) were screened from 71% of the filters and STEC was isolated from 14% of these. Of the STEC isolates, 21 contained the stx ₁ gene, 19 the stx ₂ gene, and five a combination of both stx ₁ and stx ₂ genes. Whole genome sequence typing on 34 of the 45 STEC showed that they belonged to 21 different serotypes, of which STEC O145:H28 was the most common (2%). STEC O157:H7 was only found from one (0.3%) of the filters. A combination of stx ₂ and eae genes was found from 0.7% of the total number of inline milk filters analyzed, while stx ₂ₐ was found in 24% of the whole genome‐sequenced isolates. There was a significant positive correlations between number of animals per farm and presence of pathogens on milk filters.
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