The genoprotective activity of resveratrol on aflatoxin B1-induced DNA damage in human lymphocytes in vitro
2012
Türkez, Hasan | Şişman, Turgay
Aflatoxin B1 (AFB1) has been consistently shown to be a potent mutagen and carcinogen in humans and animals. On the other hand, resveratrol (RSV), a polyphenol, has several positive biological actions such as protection of cells against DNA damage. In the present study, the antigenotoxic effect of RSV was studied against a genotoxic dose of AFB1 using the damage parameters of chromosomal aberrations (CAs) and sister chromatid exchanges (SCEs) in cultured human lymphocytes. Whole blood samples from three healthy male donors were used for this experiment and the effects of various concentrations of RSV (0, 10, 15, 25, 40, 75 and 100 µM) and AFB1 (10 µM) were tested. The results revealed that the frequencies of SCEs and CAs in lymphocytes were significantly (p < 0.05) increased by AFB1 as compared to controls. The results also showed that RSV was not genotoxic. Moreover, the number of SCEs and micronuclei induced by AFB1 could be significantly minimized by the presence of RSV. Our results suggest for the first time that RSV can antagonize the ability of AFB1 to cause DNA damage that leads to the formation of SCEs and CAs.
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