Calcium involvement in the stimulation of sex pheromone production by PBAN in the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae)
1995
Ma, P.W.K. | Roelofs, W.L.
An in vitro assay was used to study the stimulation of sex pheromone production by pheromone biosynthesis activating neuropeptide (PBAN) in female European corn borer (ECB), Ostrinia nubilalis. Synthetic Bom-PBAN was active at 0.25 nM and maximally stimulated in vitro pheromone production at 2.5 nM. With 2.5 nM Bom-PBAN, maximum pheromone production was reached within 90 min of incubation. The calcium ionophore, A23187, stimulated pheromone production independent of PBAN. Both Bom-PBAN-stimulated and ionophore-stimulated pheromone production are dependent on the presence of calcium in the incubation medium. A calcium-free incubation medium and a medium with calcium replaced by 5 mM magnesium did not support Bom-PBAN-stimulated and ionophore-stimulated pheromone production. Furthermore, a potent calcium blocker, lanthanum, inhibits Bom-PBAN stimulated pheromone production at 0.5 mM. These results suggest that the activation of sex pheromone production by PBAN in ECB is mediated, in part, by calcium ion.
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