Genetic transformation of endo-1,4-β-glucanase (Korrigan) for cellulose enhancement in Eucalyptus tereticornis

Aggarwal, Diwakar; Anil Kumar,; Sudhakara Reddy, M.

Genetic transformation of endo-1,4-β-glucanase (Korrigan) for cellulose enhancement in Eucalyptus tereticornis

2015

Aggarwal, Diwakar | Anil Kumar, | Sudhakara Reddy, M.

KORRIGAN plays an important role in cellulose biosynthesis and other physiological processes like plant growth, development, cell division and expansion. In the present study, a full length cDNA of Korrigan (PdeKOR) encoding 619 amino acids was amplified and characterized from Populus deltoides. Sequence analysis revealed the presence of glycosylation, phosphorylation sites and a single transmembrane domain (between amino acids 72 and 94) suggesting that PdeKOR belongs to GH9A subclass from glycosyl hydrolase family 9 (GH9) of proteins. The PdeKOR gene was successfully transformed into Eucalyptus tereticornis by Agrobacterium mediated genetic transformation. Efficient regeneration of transformed shoots from the explants following co-cultivation was achieved on MS medium supplemented with 25 µM α-naphthaleneacetic acid. Transgenic lines showed over expression of the PdeKOR gene as determined by qPCR analysis. Maximum expression of the PdeKOR gene was observed in the tissues of stem compared to leaves and shoot tips. This is the first report where an elite clone of E. tereticornis was successfully transformed with Korrigan gene, which can help in understanding its function in wood formation.

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