Regulation of the proliferative activity of ovarian surface epithelial cells by follicular fluid
2009
Doyle, L.K. | Donadeu, F.X.
Despite critical roles of the ovarian surface epithelium (OSE) in ovulation and post-ovulatory wound repair, little is known about the physiological mechanism regulating OSE proliferation. A role of follicles and corpora lutea in locally regulating the proliferative activity of OSE has been suggested. In this study, the effects of follicular and luteal products on proliferation of cultured OSE cells were tested using cells obtained from seasonally anoestrous ewes. Follicular fluid but not luteal extracts induced OSE cell proliferation (2.5-fold relative to untreated controls; P <0.0001). The response of OSE cells was not affected by follicle size or previous charcoal-extraction of follicular fluid (P >0.1). Treatment with IGF-1 (2.2-fold; P <0.01), EGF (1.9-fold; P <0.01) and, to a lesser extent, FSH (P <0.05) also induced OSE cell proliferation. In contrast, oestradiol or progesterone did not induce cell proliferation or enhance the effects of FSH on proliferation (P >0.1). It was concluded that follicular fluid can directly stimulate ovine OSE cell proliferation and that this effect is attributable to non-steroidal mitogens.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
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