Transcriptome-wide identification of A-to-I RNA editing sites using ICE-seq

Okada, Shunpei; Ueda, Hiroki; Noda, Yuta; Suzuki, Tsutomu

Transcriptome-wide identification of A-to-I RNA editing sites using ICE-seq

2018

Okada, Shunpei | Ueda, Hiroki | Noda, Yuta | Suzuki, Tsutomu

In A-to-I RNA editing, adenosine is converted to inosine in double-stranded regions of RNAs. Inosine, an abundant epitranscriptomic mark, contributes to a wide range of biological processes by regulating gene expression post-transcriptionally. To understand the effect of A-to-I RNA editing on regulation of the epitranscriptome, accurate mapping of inosines is necessary. To this end, we established a biochemical method called inosine chemical erasing sequencing (ICE-seq) that enables unbiased and reliable identification of A-to-I RNA editing sites throughout the transcriptome. Here, we describe our updated protocol for ICE-seq in the human transcriptome.

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