Ferrovibrio xuzhouensis sp. nov., a cyhalothrin-degrading bacterium isolated from cyhalothrin contaminated wastewater

Song, Man; Zhang, Long; Sun, Bin; Zhang, Hao; Ding, Hui; Li, Qiang; Guo, Suhui; Huang, Xing

Ferrovibrio xuzhouensis sp. nov., a cyhalothrin-degrading bacterium isolated from cyhalothrin contaminated wastewater

2015

A novel cyhalothrin-degrading strain, designated as LM-6ᵀ, was isolated from a cyhalothrin contaminated wastewater sample. The bacterium was found to be Gram stain-negative, non-spore-forming, vibrio-shaped, and motile with a single polar flagellum. Strain LM-6ᵀ was observed to grow optimally at 28–30 °C, pH 6.0 and in the absence of NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain LM-6ᵀ is a member of the genus Ferrovibrio, and showed the highest sequence similarity with Ferrovibrio denitrificans Sp-1ᵀ (97.7 %), followed by Taonella mepensis H1ᵀ (93.3 %). The major fatty acids of strain LM-6ᵀ (>5 %) were determined to be C₁₈:₁ ω7c and/or C₁₈:₁ ω6c, C₁₆:₀, C₁₈:₁ 2–OH and C₁₇:₁ iso I and/or anteiso B. The major polar lipids were identified to be phosphatidylglycerol, phosphatidylethanolamine and phosphatidylmethylethanolamine. The major respiratory quinone was determined to be ubiquinone-10. The genomic DNA G+C content of strain LM-6ᵀ is 66.5 mol %. Strain LM-6ᵀ showed low DNA–DNA relatedness with F. denitrificans Sp-1ᵀ (53.1 ± 0.5 %). On the basis of phylogenetic, genomic, phenotypic and chemotaxonomic data, strain LM-6ᵀ is considered to represent a novel species of the genus Ferrovibrio, for which the name Ferrovibrio xuzhouensis sp. nov. is proposed. The type strain is Ferrovibrio xuzhouensis LM-6ᵀ (=KCTC 42182ᵀ = ACCC 19710ᵀ).

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