2-Methylisoborneol production characteristics of Pseudanabaena sp. FACHB 1277 isolated from Xionghe Reservoir, China
2016
Zhang, Ting | Zheng, Lingling | Li, Lin | Song, Lirong
The cyanobacterium Pseudanabaena sp. FACHB 1277, a 2-methylisoborneol (2-MIB) producer isolated from Xionghe Reservoir, was identified by molecular biological methods based on the 16S rDNA sequence. Pseudanabaena sp. FACHB 1277 is a planktonic freshwater species with relatively high 2-MIB per cell density value (7.76 × 10⁻⁶ ng cell⁻¹) and specific growth rate (0.25 ± 0.01 d⁻¹). The effects of temperature and light intensity on 2-MIB production of Pseudanabaena sp. FACHB 1277 were investigated. Of the six temperatures tested, 10, 15, 20, 25, 30, and 35 °C, the maximum total 2-MIB per cell density and minimum cell density were observed at 10 °C, while the total 2-MIB and dissolved 2-MIB (including extracellular and dissolved intracellular 2-MIB) increased with increasing temperature. Among the six tested light intensities (10, 25, 40, 55, 70, and 85 μmol photons m⁻² s⁻¹), the minimum total 2-MIB per cell density and maximum cell density were observed at 25 μmol photons m⁻² s⁻¹. The total 2-MIB and extracellular 2-MIB increased with light intensity increasing from 10 to 40 μmol photons m⁻² s⁻¹, while no significant increase was observed when the light intensity was higher than 40 μmol photons m⁻² s⁻¹. The maximum intracellular 2-MIB (including dissolved and bound) occurred at 25 μmol photons m⁻² s⁻¹. The present study indicates that increasing temperature could favor the conversion of bound intracellular to dissolved 2-MIB, while increasing light intensity stimulates the release of dissolved intracellular 2-MIB into the environment.
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