The 5′ untranslated region of Bean pod mottle virus RNA2 tolerates unusually large deletions or insertions
2014
Ali, Ahmed Khamis | Lin, Junyan | Han, Junping | Ibrahim, Kadhim Mohammad | Jarjees, Mysire Majeed | Qu, Feng
Bean pod mottle virus (BPMV) is a bipartite, positive-sense (+) RNA virus of Secoviridae. We recently reported that a 137 nucleotide (nt) stretch (#263–399) of the 466nt 5′ untranslated region (5′ UTR) of BPMV RNA2 can be deleted without compromising BPMV propagation in host plants [Lin et al., J. Gen. Virol. 94 (2013) 1415–1420]. Here we demonstrate that nonviral insertions of up to 625nt is tolerated by the same region. Furthermore, one insertion mutant underwent recombination in infected plants, leading to the truncation of nt #250–361, thus extending the dispensable sequence to 150nt (nt #250–399). We are unaware of any other (+) RNA virus that tolerates insertion/deletion of these sizes (625nt/150nt) within its 5′ UTR. Importantly, tolerance of large insertions within the RNA2 5′ UTR offers a novel, more convenient site for incorporating host gene fragments, making BPMV a more versatile vector of virus-induced gene silencing.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل National Agricultural Library