Synergic degradation of diesel by Scirpus triqueter and its endophytic bacteria

Zhang, Xinying; Chen, Lisha; Liu, Xiaoyan; Wang, Chuanhua; Chen, Xueping; Xu, Gang; Deng, Kaifang

Synergic degradation of diesel by Scirpus triqueter and its endophytic bacteria

2014

The endophytic bacterium isolated from Scirpus triqueter was proved to be an oil-degraded bacterium. A pot experiment was conducted to investigate the removal ratio of diesel under the combined effect of oil-degraded microorganism (Pseudomonas sp. J4AJ) and S. triqueter. The effect of diesel on plant growth parameters, soil enzymes and microbial community was assessed after 60 days. The results showed that the soils which were planted with S. triqueter and inoculated with J4AJ displayed the highest removal ratio (54.51 ± 0.15 %) after 60-day experiment. However, the removal ratio of J4AJ-treated soils was 38.97 ± 0.55 %. Diesel was toxic to S. triqueter, as evidenced by growth inhibition during the experimental period. However, the plant height and stem biomass in the soils inoculated with J4AJ significantly increased. The combined effect of S. triqueter and J4AJ improved the enzyme activities of the catalase and dehydrogenase in the contaminated soil. The diversity index in soils under the effect of S. triqueter combined with J4AJ was lower than that of the other soil samples. The principal analysis of phospholipid fatty acid signatures revealed that the combined effect of S. triqueter and J4AJ increased the differences of soil microbial community structure with the other treatments.

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