Effects of dietary supplementation of different levels of vitamin B12 on the liver metabolism of laying hens
2022
Wang, Rui | Bai, Yan | Yang, Yu
BACKGROUND: Vitamin B₁₂ plays an important role in lipid, protein, carbohydrate and nucleic acid metabolism. We investigated the effect of supplementing layers’ diets with different vitamin B₁₂ levels on liver metabolism using a liquid chromatography–mass spectrometry‐based metabolomic approach to observe and analyse wide‐target metabolomics in the liver. RESULTS: We assigned hens to three groups, namely blank control group without vitamin B₁₂ diet (BCG), normal control group with 25 μg kg⁻¹ vitamin B₁₂ (NCG) and vitamin B₁₂ supplement group I with 100 μg kg⁻¹ vitamin (VBSG I). The VBSG I group layers had higher (P < 0.05) vitamin B₁₂ concentration than those from other groups. The egg yolk vitamin B₁₂ concentration increased (P < 0.01) with the increasing vitamin B₁₂ dietary supplemental level. Between the NCG versus BCG, VBSG I versus BCG, and VBSG I versus NCG groups, 11, 20 and 11 metabolites were significantly changed, respectively. The KEGG pathway of vitamin B₆ metabolism was significantly impacted in the NCG layers than those from BCG; seven and five pathways were significantly impacted in the VBSG I layers compared with those from BCG and NCG, including pyrimidine metabolism, vitamin B₆ metabolism, glycerophospholipid metabolism, etc. CONCLUSION: We concluded that 25 μg kg⁻¹ vitamin B₁₂ supplementation in corn–soybean meal‐based layer diet increased the egg yolk vitamin B₁₂ concentration and impacted the vitamin B₆ metabolic pathway, and 100 μg kg⁻¹ of it increased the egg yolk and liver vitamin B₁₂ concentrations and impacted vitamin B₆, lipid, nucleic acid and amino acid metabolic pathways. © 2022 Society of Chemical Industry.
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