Development of a rapid and simple HPLC-UV method for determination of gallic acid in Schinopsis brasiliensis
2015
Fernandes, Felipe H.A. | Batista, Rayanne S. de A. | de Medeiros, Francinalva D. | Santos, Fábio S. | Medeiros, Ana C.D.
The aim of this work was to develop and validate an analytical method for the identification of the chemical marker of Schinopsis brasiliensis Engl., Anacardiaceae. It would determine the total polyphenols and flavonoid content by spectrophotometric methodology in the dried extract of plant. The chromatographic profiles of S. brasiliensis were determined using HPLC-UV. The liquid chromatography method was conducted on a Phenomenex Gemini NX C18 column (250×4.6mm, 5μm). The mobile phase consisted of 0.05% orthophosphoric acid: methanol. The flow rate was 1ml/min and effluents were monitored at 271nm. The retention time for gallic acid was 8.5min. The described method has the advantage of being both rapid and easy. Hence it can be applied for routine quality control analysis of herbal preparation containing S. brasiliensis.
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