Chromosomal fragment responsible for genetic mosaicism in larval body marking of the silkworm, Bombyx mori
1991
Fujiwara, H. | Ninaki, O. | Kobayashi, M. | Kusuda, J. | Maekawa, H.
Several genetic mosaics for larval body marking of the silkworm, Bombyx mori, have been induced by X-ray irradiation. It is hypothesized that the occasional loss of chromosomal fragments carrying the genes for body marking during development may give rise to this type of mosaicism. Using pulsed field gel electrophoresis (PFGE), we find that a DNA molecule of about 2.5 megabases (Mb) is present in one type of mosaic (mottled striped strains pSm788 and pSm872), and not in any other strain. This DNA fragment hybridizes strongly with some chorion genes which are less than 6.9 cM away from the pS locus, and hence it corresponds to a chromosomal fragment containing genes for both striped marking (pS) and the chorion. In the non-mottled pS strain, the phenotype before X-ray irradiation, no band was detected either on a PFGE gel or after hybridization with the chorion probe. These results suggest that the mottled pS strains carry short chromosome fragments which are lost differentially during cell divisions.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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