Inactivation of MS2 coliphage by UV and hydrogen peroxide: Comparison by cultural and molecular methodologies
2014
Sherchan, Samendra P. | Snyder, Shane A. | Gerba, Charles P. | Pepper, Ian L.
The use of advanced oxidation processes (AOP) are expected to increase for removal of emerging contaminants and pathogens from drinking water. In this study, the performance of a small community ultraviolet light reactor in combination with hydrogen peroxide (H ₂O ₂) for MS2 coliphage inactivation with two different flow rate conditions of 1 gal/min (gpm) and 2 gpm was evaluated. Following UV radiation, MS2 showed a reduction of 5.3–5.8 log ₁₀ when quantified with cultural plaque counts, whereas corresponding quantitative polymerase chain reaction (qPCR) data showed only a 1.7–2.8 log ₁₀ reduction in viral RNA copy number. When H ₂O ₂ was added at either 2.5 or 5 ppm with UV at both flow rate conditions, enhanced MS2 inactivation occurred with a more than 7 log ₁₀ reduction observed via plaque counts, indicating that all added MS2 had been inactivated, since no plaques were formed after incubation at 37°C for 24 h. In contrast, qPCR only showed a corresponding 3–4 log ₁₀ reduction in viral RNA copy number. This research also sheds light on the inactivation of MS2 with ultraviolet light and in the presence of hydroxyl radicals and provides a practical use of qPCR to detect MS2 concentration following advanced oxidation relative to traditional plaque methodology; however qPCR detection overestimates the true number of infective virus.
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