<b>QTL mapping and genetic analysis of inhibitory effect of lysine on post-germination growth and seedling establishment of maize</b>
2006
Anzala, F. | Mor?re-Le Paven, M.-C. | Birolleau-Touchard, C. | Giauffret, C. | Limami, A. M.
Radicle elongation that allows rhizosphere colonization was used as a marker of seedling establishment. The goal of the present work was to determine the genetic basis of the effect of the aspartate family pathway, in particular lysine, on the radicle elongation of maize seedlings. For this purpose a population derived from an advanced backcross between a flint European line F2 and a highland tropical line F334 as the donor parent, was used for mapping QTLs related to the effect of lysine on radicle elongation. The parental lines showed contrasting germination efficiency and root elongation under both control conditions (imbibition on water at 20°C) and on medium supplied with lysine. Two QTLs for radicle elongation under control conditions were located on chromosome 2 (136 cM) near the marker bnlg1721 and on chromosome 5 (146 cM) near the marker umc1792. These QTLs explained 9.4% and 10.5%, respectively, of the phenotypic variability for radicle elongation. When germination was carried out on medium containing 5 mM lysine, three QTLs for radicle elongation were located on chromosome 7 (90 cM) near the marker umc1112, on chromosome 10 (42 cM) near the marker bnlg1037 and on chromosome 10 (68 cM) near the marker umc1053; these QTLs explained 12.0%, 12.3% and 12.4%, respectively, of the phenotypic variability for radicle elongation. Irrespective of the germination and post-germination medium, favourable alleles for all detected QTLs were associated with parental line F334.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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