Degradation of starch by incubation with rumen fluid. A comparison of different starch sources
1989
Cone, J.W. | Cline-heil, W. | Malestein, A. | Klooster, A.T. van't
The degradability of starch from various feedstuffs was investigated in vitro by incubation of 500-mg amounts in 50 ml of a 3:1 rumen fluid/buffer solution at 39 degrees C for 6 h. The rumen fluid was obtained from one of three cows fed on hay or hay and concentrate. The degree of degradation after 6 h incubation varied strongly for the 23 feedstuffs investigated. The degradation of starch from the same feedstuff in rumen fluid from a hay-fed cow was significantly lower than in rumen fluid from a concentrate-fed cow. It seemed that differences in degradability between feedstuffs were not determined by the ration of the donor cow, but merely by the properties of the starch. Processed feedstuffs showed a higher degradation of their starch than the unprocessed feedstuffs, independent of the ration of the donor cow. Particle size influenced degradation, but not of the starch of tapioca meal. A fairly constant ranking in degradability between the various feedstuffs was found. Fermentation of mixtures of feestuffs showed about the same rate of degradation as found for the single products. Only when great differences in the degree of degradability existed was the degradation of the total starch enhanced. The time of collection of rumen fluid strongly influenced the in-vitro degradation of starch.
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