Establishment of embryogenic cultures and determination of their bioactive properties in Rosa rugosa
2016
Jang, Hye-Rim | Lee, Hyun-Jeong | Park, Byung-Jun | Pee, Ok-Ja | Paek, Kee-Yoeup | Pak, So-yŏng
Somatic embryogenesis represents a powerful in vitro plant culture system and provides high potential for the production of biomass in woody plants. Rosa rugosa is known not only for its ornamental value, but also as a source of antioxidants with the potential to be used as ingredients for industrial products such as cosmetics. The present study was conducted to investigate the effects of hormonal concentration on embryogenic callus (EC) induction from petal and leaf segments of this species. The effect of selected cell lines on the production of total phenolics and flavonoids in R. rugosa was also studied. The highest percentage of EC (14.1 ± 3.9%) and root formation (20.3 ± 6.9%) was observed in the presence of 45.0 μM of 2,4-D. Among the three studied lines, the highest total phenolics and flavonoid levels were observed in H-1 (17.71 mg·g⁻¹ DW), while H-18 contained the lowest. Histological studies of the H-1 and H-18 cell lines revealed differences in polyphenol accumulation and polysaccharides. The effect of developmental stage on growth, shoot, and root regeneration, as well as on phenolic and flavonoid content, was also studied in H-18. The present study indicates that selection of the proper type and concentration of hormone is essential for EC induction from petals in R. rugosa. Moreover, the accumulation of bioactive compounds was found to greatly depend on the genotype. The results of this study are useful for the production of selected antioxidants by somatic embryos from R. rugosa petals.
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