Antifungal activity of Ziziphora clinopodioides Lam. essential oil against Sclerotinia sclerotiorum on rapeseed plants (Brassica campestris L.)
2016
Ma, Bing-Xin | Ban, Xiao-Quan | He, Jing-Sheng | Huang, Bo | Zeng, Hong | Tian, Jun | Chen, Yu-Xin | Wang, You-Wei
The present study aimed to determine the in vitro and in vivo effects of Ziziphora clinopodioides Lam. essential oil (ZCEO) against Sclerotinia sclerotiorum on rapeseed plants. Gas chromatography-mass spectrometry (GC-MS) detected 21 compounds that represented 98.3% of the total amount of extracted oil, which mainly comprised pulegone (53.5%), isomenthone (10.4%), and carvone (5.7%). The mycelial growth of S. sclerotiorum was completely inhibited at essential oil concentrations of 1.25 and 0.15 μl ml−1 under contact and vapor phase conditions, respectively. Under contact phase, the germination of sclerotial was inhibited at the concentration of 1.00 μl ml−1. The essential oil concentration of 0.15 μl ml−1 in the vapor phase showed a strong inhibitory effect on sclerotial germination. Sclerotinia sclerotiorum growth on detached rapeseed leaves and potted rapeseed plants were dose dependently inhibited by the essential oil. Considerable morphological changes were also observed in the fungal hyphae and sclerotia. Both in vitro and in vivo results indicated ZCEO can effectively inhibit the growth of S. sclerotiorum. Thus, ZCEO could be used for crop protection.
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