The amphiphilic hydrophobin Vmh2 plays a key role in one step synthesis of hybrid protein–gold nanoparticles
2015
Politi, Jane | De Stefano, Luca | Longobardi, Sara | Giardina, Paola | Rea, Ilaria | Methivier, Christophe | Pradier, Claire-Marie | Casale, Sandra | Spadavecchia, Jolanda
We report a simple and original method to synthesize gold nanoparticles in which a fungal protein, the hydrophobin Vmh2 from Pleurotus ostreatus and dicarboxylic acid-terminated polyethylene-glycol (PEG) has been used as additional components in a one step process, leading to hybrid protein–metal nanoparticles (NPs). The nanoparticles have been characterized by ultra-violet/visible, infrared and X-ray photoelectron spectroscopies, dynamic light scattering and also by electron microscopy imaging. The results of these analytical techniques highlight nanometric sized, stable, hybrid complexes of about 12nm, with outer surface rich in functional chemical groups. Interaction with protein and antibodies has also been exploited.
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