Molecular characterization and functional analysis of Cry toxin receptor‐like genes from the model insect Galleria mellonella
2022
Dutta, Tushar K. | Veeresh, Arudhimath | Phani, Victor | Kundu, Artha | Santhoshkumar, Kodhandaraman | Mathur, Chetna | Sagar, Doddachowdappa | Sreevathsa, Rohini
Crystal (Cry) toxins produced from the soil bacterium, Bacillus thuringiensis (Bt), have gained worldwide attention for long due to their insecticidal potential. A number of receptor proteins located on the epithelial cells of the larval midgut were shown to be crucial for Cry intoxication in different insect pests belonging to order Lepidoptera, Diptera and Coleoptera. A beehive pest, Galleria mellonella, serves as an excellent insect model for biochemical research. However, information on the Cry receptor‐like genes in G. mellonella is limited. In the present study, the full‐length sequences of four putative Cry receptor genes (ABC transporter, alkaline phosphatase, aminopeptidase N and cadherin) were cloned from G. mellonella. All these receptor genes were substantially upregulated in the midgut tissue of fourth‐instar G. mellonella larvae upon early exposure (6 h) to a sub‐lethal dose of Cry1AcF toxin. Oral and independent delivery of bacterially‐expressed dsRNAs corresponding to four receptor genes in G. mellonella suppressed the transcription of target receptors which in turn significantly reduced the larval sensitivity to Cry1AcF toxin. As the laboratory populations of G. mellonella develop Bt resistance in a relatively short time, molecular characterization of Cry receptor genes in G. mellonella performed in the present study may provide some useful information for future research related to the genetic basis of Bt resistance in the model insect.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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