Antioxidant content and identification of phenolic/flavonoid compounds in the pollen of fourteen plants using HPLC-DAD
2020
Bakour, Meryem | Campos, Maria da Graça | Imtara, Hamada | Lyoussi, Badiâa
This study was carried out to evaluate the in vitro potential antioxidant activity, total phenolics, flavones, and flavonol content of hydro-ethanolic extracts of pollen from fourteen plants belonging to ten families. The identification of phenolic and flavonoid compounds was accomplished using HPLC-DAD analysis. The results of the total phenolic contents varied from 9.20 ± 0.12 mg GAE (Gallic acid equivalent)/g in the pollen sample of Malva sylvestris, to 71.20 ± 0.72 mg GAE (Gallic acid equivalent)/g in the pollen of Mentha spicata, while flavones and flavonols content ranged from 1.27 ± 0.07 mg QE (Quercetin equivalent)/g in the pollen of Scolymus hispanicus to 15.44 ± 1.14 mg QE (Quercetin equivalent)/g in the pollen samples collected from Anacyclus radiatus. The total antioxidant capacity assessed using the molybdate assay ranged between 17.84 ± 1.03 mg AAE (ascorbic acid equivalent)/g in Malva sylvestris to 99.54 ± 0.90 mg AAE (ascorbic acid equivalent)/g in Capparis spinosa pollen. Concerning antioxidant ability, Mentha spicata and Mentha pulegium had the best antioxidant ability. The HPLC-DAD analysis showed a wide variety of flavonols and flavones, mainly glycosides of apigenin, luteolin, kaempferol, quercetin, and isorhamnetin. The phenolic acids are represented by derivatives of caffeic acid, p-hydroxybenzoic acid, and vanillic acid. In Malva sylvestris and Scolymus hispanicus some compounds found could be alkaloids, however, in this work the confirmation of such chemical group was not carried out but it will be included in further studies.
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