Physicochemical and Biochemical Properties of Pepsin‐Solubilized Collagen Isolated from the Integument of Sea Cucumber (Stichopus vastus)
2014
Abedin, Md Zainul | Karim, Alias A. | Latiff, Aishah A. | Gan, Chee‐Yuen | Che Ghazali, Farid | Zzaman, Wahidu | Hossain, Md Murad | Faiyaz, Ahmed | Absar, Nurul | Sarker, Md Zaidul Islam
The integument (high collagen content) of sea cucumber Stichopus vastus is generally wasted after harvesting, whereas only its stomach and intestines are eaten in few Asian countries. Amino acid composition, thermal transition temperature (Tm), zeta potential, solubility, moisture absorption and retention capacities, proximate composition and morphology of pepsin‐solubilized collagen (PSC) isolated from the integument of S. vastus were studied. Amino acid composition revealed that glycine was dominant in the isolated collagen. PSC was found to have an isoelectric pH of 4.67, good moisture absorption and retention capacity at higher humidity, a sharp effect of pH and NaCl concentration on solubility, and an inverse relationship between temperature and viscosity. PSC also showed the maximum Tm to be 37.3C, very high protein content and ultrastructural characteristics. Hence, the PSC has the potential to be used as a functional ingredient in food, cosmetics and nutraceutical products. PRACTICAL APPLICATIONS: Sea cucumber S. vastus is an underutilized resource as most of its parts including integument, which is highly rich in collagen, are discarded as waste except its stomach and intestines, which are consumed as salad. At present, land‐based mammals are the main sources of industrial collagen, which has a risk of viral disease transmission and religious disputation, whereas marine sources are free from such criticisms. Besides, collagen isolated from such waste might have industrial applications. The purpose of this research is to investigate physicochemical and biochemical properties of PSC isolated from the integument of S. vastus for exploring the potential applications in processing of different industrial products as an alternative to land‐based origin.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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