Effect of dietary protein levels on reproductive development and distribution of amino acids in the body tissues of female Pangasianodon hypophthalmus (Sauvage, 1878) broodstock in captivity
2015
Kabir, Muhammad Anamul | Ghaedi, Alireza | Talpur, Allah Dad | Hashim, Roshada
The present study investigated the optimum dietary protein level for the maturation of adult Pangasianodon hypophthalmus broodstock. Four isocaloric diets containing 250, 300, 350 and 400 g kg⁻¹ of protein levels were prepared and presented to triplicate groups of fish. The fish (mean weight 770 ± 17.23 g and 712 ± 23.42 g for females and males respectively) were stocked in outdoor canvas tanks (4 m × 1 m × 1 m) at a stocking density of 20 fish/tank with a male: female ratio of 1:4. The fish were fed the test diets to satiation twice daily for 6 months. Gonadosomatic index (GSI) and fecundity were similar among fish fed dietary protein levels, higher than those fed on the 250 g kg⁻¹. Final weight, weight gain, oocyte weight were significantly highest (P < 0.05) for the fish fed 350 and 400 g kg⁻¹ dietary protein treatments. Only the 350 g kg⁻¹ dietary protein treatment resulted in significantly best ovipositor diameter and % ripe egg. Amino acid levels were highest in the muscle followed by the oocyte and liver of fish fed 350 and 400 g kg⁻¹ dietary protein levels. The present results suggested that a dietary protein level of 350 g kg⁻¹ can be recommended for the development of P. hypophthalmus broodstock.
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