RETRACTION: Isotopic analysis of N and O in NO3 − by selective bacterial reduction to N2O for groundwater pollution
2014
Fang, Jingjing | Ma, Chuanming | Liu, Cunfu | Yue, Xiangbing
We describe a method to determine the nitrogen and oxygen isotopic composition of nitrate in groundwater samples (¹⁵N/¹⁴N and ¹⁸O/¹⁶O, respectively), which is based on the analysis of nitrous oxide gas (N₂O) that is produced quantitatively from nitrate by denitrifying bacteria. This method which is simple, inexpensive and effective in the removal of nitrite is greatly selective for NO₂ ⁻ and was used for mixed samples containing both NO₂ ⁻ and NO₃ ⁻ with little or no measurable cross-contamination. The precision of δ¹⁵N and δ¹⁸O are 0.3 and 0.17 ‰ respectively, compared to that of 0.1 and 0.5 ‰ abroad (Brand et al. in Org Geochem 21:585–594, 1994; Begley and Scrimgeour in Anal Chem 69(8): 1530–1535, 1997; Kornexl et al. in Rapid Commun Mass Spectrom 13(16):1685–1693, 1999; Böhlke et al. in Rapid Commun Mass Spectrom 17:1835–1846, 2003; Gehre and Strauch in Rapid Commun Mass Spectrom 17(13):1497–1503, 2003; Werner in Isot Environ Health Stud 39:85–104, 2003).
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